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Apparently enough of it will kill them. This was trying to use it to kill off black widows on produce, not in a garage:
https://mbao.org/static/docs/confs/2005-sandiego/papers/065LeeschJ%20THE%20TOXICITY%20OF%20OZONE%20TO%20BLACK%20WIDOW%20SPIDERS.pdf
But I dunno if it's the best pesticide to use. I know that ozone is pretty reactive and can damage some substances. I remember rubber being one.
kagis
https://www.nrc.gov/docs/ML1820/ML18207A768.pdf
Looks like it's also not great for steel, zinc, nylon, and some other stuff.
EDIT: Note that they were using 12,000 ppm for 1 hour to achieve 100% elimination of black widows. It sounds like it'd be extremely hazardous to be working with that kind of concentration.
https://www.cdc.gov/niosh/idlh/10028156.html
So that's something like 240 times the concentration to kill a human in that period of time. Even if one can get the hardware to produce that kind of concentration in a garage, I wouldn't want to do so.