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Carbon monoxide would be cheaper. We used it for euthanizing animals that couldn't be saved at the wildlife rehab center I worked at. Though, it was done with sealed induction box, not a drafty barn like someone mentioned
Sounds like it would be more expensive? Nitrogen is incredibly cheap to concentrate out of the air, 70% of what we breath is nitrogen after all.
Monoxide is incredibly cheap to produce with a crappy farm truck or old tractor. You doing need to distill or concentrate anything, just a hose and the exhaust pipe and a couple hours of fuel for idling.
We used it to gas a nest of rats that had settled in under a grain bin floor. Only a couple rats popped out and they were dazed, the dogs quickly snacked them up. The rest expired rapidly.
A chicken barn is big and drafty but you could just use multiple tractors or detune them on purpose. Any engine running rich produces a lot of CO.