this post was submitted on 18 Jul 2023
1 points (100.0% liked)

Image Processing and Analysis

94 readers
3 users here now

A community for sharing knowledge (interesting or new methods, scripts, theoretical concepts, etc), asking questions (troubleshooting, asking for suggestions, general concepts, etc) and discussing all topics related to image processing and analysis (show and tell, careers, software, etc).

founded 1 year ago
MODERATORS
 

I don't really use QuPath, I don't like it. But the person before me used it to annotate images and now I need to transfer these ROIs/Annotations to ImageJ where my pipeline is. Managed to find a good script for exporting with annotations, but it seems this person transformed the images (differently each time fml), and I need them to be the right orientation because I'm transferring those annotations to another set of images of the same samples from another microscope. Needless to say, I need to do some image registration, a very easy task /s. So is there any way to at least revert back to the initial orientation before export so I make the registration easier? I'm using SIFT which isn't really capable of accounting for mirroring/flipping, so that step really needs to be fixed beforehand. Any ideas? Or at the very least, is there a way to extract transformation history from the Metadata or so such that I can at least use that info to programmatically revert in ImageJ?

no comments (yet)
sorted by: hot top controversial new old
there doesn't seem to be anything here