this post was submitted on 14 Nov 2024
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What do they do?
Top left is a thermal cycler. Basically it heats and cools samples at a given rate. This is primarily used for generic PCR, and certain enzymatic reactions. Top right is the fancier version of this, it is for qPCR, so it can do the heating and cooling and has a laser/detector for the dye or probe that reacts to generating more dna with each PCR cycle so you can quantify approximately how much of the target DNA you had.
Bottom right is a luminex. This uses detection of fluorophore signals to measure multiple analyates, usually different proteins.
Idk what bottom left is.
Top left is the CFX96/384, which is also a qPCR instrument.
Bottom left is the 3500 Genetic Analyzer, as someone identified. It's used for sanger sequencing I believe. My last lab had one but I was never trained on it.
I thought it was just their T100, the CFX96s I've used don't have the touchscreen but yeah the bigger "lid" does look like for the CFX.
The bases and hot blocks are interchangable with that series. We recently upgraded to the Opus but our previous CFX96 had the T1000 touch base.